CHAPEL HILL, N.C. – A new look at a 425-year-old map has yielded a tantalizing clue about the fate of the Lost Colony, the settlers who disappeared from North Carolina’s Roanoke Island in the late 16th century.

Experts from the First Colony Foundation and the British Museum in London discussed their findings Thursday at a scholarly meeting on the campus of the University of North Carolina at Chapel Hill. Their focus: the “Virginea Pars” map of Virginia and North Carolina created by explorer John White in the 1580s and owned by the British Museum since 1866.

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Image shows the map named “La Virginea Pars” painted by explorer John White in the 1580s depicting the North Carolina coast from Chesapeake Bay to Cape Lookout. The Associated Press

“We believe that this evidence provides conclusive proof that they moved westward up the Albemarle Sound to the confluence of the Chowan and Roanoke rivers,” said James Horn, vice president of research and historical interpretation at the Colonial Williamsburg Foundation and author of a 2010 book about the Lost Colony.

“Their intention was to create a settlement. And this is what we believe we are looking at with this symbol — their clear intention, marked on the map …”

Attached to the map are two patches. One patch appears to merely correct a mistake on the map, but the other — in what is modern-day Bertie County in northeastern North Carolina — hides what appears to be a fort. Another symbol, appearing to be the faint image of a different kind of fort, is drawn on top of the patch.

The American and British scholars believe the fort symbol could indicate where the settlers went. The British researchers joined the Thursday meeting via webcast.

In a joint announcement, the museums said, “First Colony Foundation researchers believe that it could mark, literally and symbolically, ‘the way to Jamestown.’ As such, it is a unique discovery of the first importance.”

White made the map and other drawings when he traveled to Roanoke Island in 1585 on an expedition commanded by Sir Ralph Lane. In 1587, a second colony of 116 English settlers landed on Roanoke Island, led by White. He left the island for England for more supplies but couldn’t return again until 1590 because of the war between England and Spain.

When he came back, the colony was gone. White knew the majority had planned to move “50 miles into the maine,” as he wrote, referring to the mainland. The only clue he found about the fate of the other two dozen was the word “CROATOAN” carved into a post, leading historians to believe they moved south to live with American Indians on what’s now Hatteras Island.

But the discovery of the fort symbol offers the first new clue in centuries about what happened to the 95 or so settlers. Researchers at the British Museum discovered it because Brent Lane, a member of the board of the First Colony Foundation, asked a seemingly obvious question: What’s under those two patches?

Researchers say the patches attached to White’s excruciatingly accurate map were made with ink and paper contemporaneous with the rest of the map. One corrected mistakes on the shoreline of the Pamlico River and the placing of some villages. But the other covered the possible fort symbol, which is visible only when the map is viewed in a light box.

The map was critical to Sir Walter Raleigh’s quest to attract investors in his second colony; critical to his convincing Queen Elizabeth I to let him keep his charter to establish a colony in the New World, and critical to the colonists who navigated small boats in rough waters.

So that made Lane wonder: “If this was such an accurate map and it was so critical to their mission, why in the world did it have patches on it? This important document was being shown to investors and royalty to document the success of this mission. And it had patches on it like a hand-me-down.”

Researchers don’t know why someone covered the symbol with a patch, although Horn said the two drawings could indicate the settlers planned to build more of a settlement than just a fort.

Excavating the site, which is privately owned, won’t begin anytime soon. But it doesn’t have to, said Nicholas Luccketti, a professional archaeologist in Virginia and North Carolina for more than 35 years.

Archaeologists must first re-examine ceramics, including some recovered from an area in Bertie County called Salmon Creek, he said.

“This clue is certainly the most significant in pointing where a search should continue,” Lane said. “The search for the colonists didn’t start this decade; it didn’t start this century. It started as soon as they were found to be absent from Roanoke Island … I would say every generation in the last 400 years has taken this search on.”

But none have had today’s sophisticated technology to help, he said. “None of them had this clue on this map.”

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