CARACAS, Venezuela — Opponents of President Nicolas Maduro at home and abroad tried again Wednesday to pressure the socialist leader into halting his plans to rewrite Venezuela’s constitution, though there was no public sign their efforts were working.

The Trump administration announced sanctions on 13 current and former members of Maduro’s administration, freezing their U.S. assets and barring Americans from doing business with them. The U.S. also joined with a dozen other regional governments in urging Maduro to suspend Sunday’s election of a national assembly for rewriting the charter.

Those moves came as a coalition of Venezuelan opposition groups organized a second national strike in a week. Millions observed the 48-hour strike, and activists threw up roadblocks in many neighborhoods to keep others from getting to work. One person was reported killed.