JEBALIYA REFUGEE CAMP, Gaza Strip — Israel’s defense minister said Saturday that Hamas leaders are the “only culprits” in continued bloodshed on the Gaza border, as the European Union and a top United Nations official made new demands to investigate shootings of unarmed Palestinians by Israeli soldiers, including the killing of a 14-year-old boy.

Avigdor Lieberman’s comments on Twitter came a day after four Palestinians, including the teenager, were shot and killed by Israeli army fire from across the border fence, according to Gaza health officials.

More than 150 Palestinians were wounded Friday, in the fourth round of weekly Hamas-led mass protests in the border area.

The teenager, Mohammed Ayyoub, was about 150 yards from the fence when he was hit, Gaza photographer Abed Alhakeem Abu Rish said. He said the boy was about to take cover when he was shot and fell to the ground. The Israeli military said it is investigating the incident.

The latest deaths brought to 32 the number of Palestinians killed by Israeli troops in protests since late March. More than 1,600 have been wounded by live rounds in the past three weeks, according to the Gaza Health Ministry.

The rising Palestinian casualty toll signaled that Israel’s military is sticking to its open-fire rules despite international criticism of the use of lethal force against unarmed protesters.

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Israel has said it is defending its sovereign border, including Israeli communities on the other side. It has alleged that Hamas uses the protests as cover for attacks and for damaging the border fence.

Late Saturday, the military released photos and videos depicting what it has said it is trying to counter. In one image, young boys, under the cover of smoke, are seen charging the fence and uprooting part of it to allow for an infiltration.

The military said it had distributed leaflets warning the residents of Gaza to stay away from the fence but that “the terror organizations in the Gaza Strip continue to exploit civilians, including many youth and children, while putting them in unnecessary danger.”

Rights groups have said Israel’s open-fire orders are unlawful because they effectively allow soldiers to open fire on unarmed demonstrators.

The marches are part of what organizers have billed as an escalating showdown with Israel, to culminate in a mass march on May 15.

The top Hamas leader, Ismail Haniyeh, said Friday that people should get ready for large crowds spilling across the border that day.

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“Our people will outnumber the occupation and force it from our land,” he said, referring to Israel.

The latest killings sparked new demands by a senior U.N. official and by the E.U. for an investigation.

Nikolay Mladenov, a top U.N. envoy in the region, said on Twitter that it is “outrageous to shoot at children.”

The European Union urged the Israeli military to “refrain from using lethal force against unarmed protesters.”

Mohammed Ayyoub’s father, Ibrahim, said Saturday that his son was killed in cold blood.

“Mohammed did not deserve to be executed,” the 42-year-old said as mourners streamed to the family’s shack in the Jebaliya refugee camp. “He was not holding a stone or a gun … Mohammed was not running toward the fence.”

The Israeli military has repeatedly warned that it will not allow protesters to get close to or damage the fence.

The Israeli defense minister, said on Twitter that “the only culprits in the death of the … boy in Gaza are Hamas leaders.”

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