DONETSK, Ukraine —Pro-Russian militants, boasting that they did not take orders from diplomats in Washington or Moscow, refused to end their armed occupation of a dozen government buildings across eastern Ukraine on Friday, upending hopes for a quick end to the standoff.

The defiance came just hours after Russia, the European Union, Ukraine and the United States sought to de-escalate the conflict with an agreement signed in Geneva that urged restraint on all sides, and called on the pro-Russia activists to lay down their baseball bats and molotov cocktails and walk away from their barricades at the city halls and police stations.

Additional Photos

A masked activist walks among barricades Friday at an occupied building in Donetsk, Ukraine. Militants are calling for the interim government in Kiev to resign. The Associated Press

At a news conference Friday on the top floor of the regional government offices they stormed last weekend, Denis Pushilin, a leader of a group calling itself the Donetsk People’s Republic, said he and his men had no intention of abandoning their positions as long as the new government in Kiev still stood.

“It is an illegal junta,” said Anatoliy Onischenko, another separatist leader, of the Kiev government. “They should leave their buildings first.”

With young men in black balaclava masks over their faces standing behind him, Pushilin said that nobody from the pro-Russia groups in Ukraine were at the negotiating table in Geneva, and because they were not consulted, they had no obligation to do anything.

The Donetsk People’s Republic flag, sporting a Russian-style eagle, flew on top of the building. The protesters were camped in the offices and sprawled on the floors. Water came from fire hoses; the cafeteria was brimming with donated food, and someone had set up a makeshift infirmary.

The central government appeared to take a conciliatory approach late Friday, when in a joint televised address, acting President Oleksander Turchinov and Prime Minister Arseny Yatseniuk called for national unity and urged people to refrain from violence over the Easter weekend.

Both men said they would support constitutional change to decentralize power and allow for more local control, giving regional governments their pick of an official language – a central demand of Russian-speaking protesters in the east.

“The Ukrainian government is prepared to conduct comprehensive constitutional reform which will strengthen the powers of the regions,” “We will strengthen the special status of the Russian language and protect this language,” Yatseniuk said.

One of the new government’s first acts in parliament after ousting former president Viktor Yanukovych in February was to deny regional governments the power to make Russian an official language. The legislation was later vetoed, but the damage had been done in the eyes of Russian speakers, who feared second-class citizenship in the new order.

In another sign that Kiev is searching for compromise that would calm pro-Russia activists, the Ukrainian presidential candidate Yulia Tymoshenko, who had been imprisoned by the Yanukovych government, made a surprise appearance Friday in Donetsk, vowing to negotiate with the break-away protesters.

“I want myself to understand their demands, what they expect, whose interests they represent,” she said. “I hope these negotiations will serve a constructive role and that we can find a way to restore harmony between the west and east of Ukraine.”

The Ukraine prime minister said Friday that parliament was ready to pass a bill that would grant amnesty to protesters who vacate occupied buildings and put down their weapons.

But he said his government did not harbor “unreasonable” expectations that the stalemate would quickly end.

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