DONETSK, Ukraine – The Interior Ministry headquarters in eastern Ukraine’s largest city fell to pro-Russia separatists Tuesday after a five-hour gunbattle that erupted hours after the Ukrainian president ended a cease-fire.

The shaky cease-fire had given European leaders 10 days to search for a peaceful settlement, and its end raised the prospect that fighting could flare with new intensity in a conflict that has already killed more than 400 people since April.

In Tuesday’s clashes, rebels fought for more ground, and badly trained and disorganized government troops seemed incapable of crushing the mutiny.

President Petro Poroshenko had called a unilateral cease-fire to try to persuade the rebels to lay down their weapons and hold peace talks. Some of the rebels signed onto the break in fighting as tentative negotiations began, but each side accused the other of repeated violations. When he ended the cease-fire, the president said the rebels were not serious about peace.

In Moscow, Russian President Vladimir Putin argued that substantive talks with representatives in eastern Ukraine had failed to start in earnest and that the cease-fire announced by Poroshenko amounted to an ultimatum to the rebels to disarm.

The Russian leader also denounced the Western threat of sanctions as blackmail

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